Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response.
Identifieur interne : 000D63 ( Main/Exploration ); précédent : 000D62; suivant : 000D64Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response.
Auteurs : Oystein Angen [Danemark] ; John Thomsen ; Lars Erik Larsen ; Jesper Larsen ; Branko Kokotovic ; Peter M H. Heegaard ; Jörg M D. EnemarkSource :
- Veterinary microbiology [ 0378-1135 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Bovins, Haptoglobines (métabolisme), Infections bactériennes (diagnostic), Infections bactériennes (médecine vétérinaire), Liquide de lavage bronchoalvéolaire (microbiologie), Maladies des bovins (diagnostic), Maladies des bovins (microbiologie), Maladies des bovins (virologie), Maladies virales (diagnostic), Maladies virales (médecine vétérinaire), Pneumopathie infectieuse (diagnostic), Pneumopathie infectieuse (microbiologie), Pneumopathie infectieuse (médecine vétérinaire), Pneumopathie infectieuse (virologie), Poumon (microbiologie), Poumon (virologie), Protéine amyloïde A sérique (métabolisme), Protéine de la phase aigüe (métabolisme), Réaction de polymérisation en chaîne (médecine vétérinaire), Trachée.
- MESH :
- diagnostic : Infections bactériennes, Maladies des bovins, Maladies virales, Pneumopathie infectieuse.
- microbiologie : Liquide de lavage bronchoalvéolaire, Maladies des bovins, Pneumopathie infectieuse, Poumon.
- médecine vétérinaire : Infections bactériennes, Maladies virales, Pneumopathie infectieuse, Réaction de polymérisation en chaîne.
- métabolisme : Haptoglobines, Protéine amyloïde A sérique, Protéine de la phase aigüe.
- virologie : Maladies des bovins, Pneumopathie infectieuse, Poumon.
- Animaux, Bovins, Trachée.
English descriptors
- KwdEn :
- Acute-Phase Proteins (metabolism), Animals, Bacterial Infections (diagnosis), Bacterial Infections (veterinary), Bronchoalveolar Lavage Fluid (microbiology), Cattle, Cattle Diseases (diagnosis), Cattle Diseases (microbiology), Cattle Diseases (virology), Haptoglobins (metabolism), Lung (microbiology), Lung (virology), Pneumonia (diagnosis), Pneumonia (microbiology), Pneumonia (veterinary), Pneumonia (virology), Polymerase Chain Reaction (veterinary), Serum Amyloid A Protein (metabolism), Trachea, Virus Diseases (diagnosis), Virus Diseases (veterinary).
- MESH :
- chemical , metabolism : Acute-Phase Proteins, Haptoglobins, Serum Amyloid A Protein.
- diagnosis : Bacterial Infections, Cattle Diseases, Pneumonia, Virus Diseases.
- microbiology : Bronchoalveolar Lavage Fluid, Cattle Diseases, Lung, Pneumonia.
- veterinary : Bacterial Infections, Pneumonia, Polymerase Chain Reaction, Virus Diseases.
- virology : Cattle Diseases, Lung, Pneumonia.
- Animals, Cattle, Trachea.
Abstract
Trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during September and November 2002. The 90 samples were cultivated and investigated by PCR tests targeting the species Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, Mycoplasma dispar, and Mycoplasma bovirhinis. A PCR test amplifying the lktC-artJ intergenic region was evaluated and shown to be specific for the two species M. haemolytica and Mannheimia glucosida. All 90 aspirations were also analyzed for bovine respiratory syncytial virus (BRSV), parainfluenza-3 virus, and bovine corona virus by antigen ELISA. Surprisingly, 63% of the apparently healthy calves harbored potentially pathogenic bacteria in the lower respiratory tract, 60% of these samples contained either pure cultures or many pathogenic bacteria in mixed culture. Among diseased calves, all samples showed growth of pathogenic bacteria in the lower respiratory tract. All of these were classified as pure culture or many pathogenic bacteria in mixed culture. A higher percentage of the samples were positive for all bacterial species in the group of diseased animals compared to the clinically healthy animals, however this difference was only significant for M. dispar and M. bovirhinis. M. bovis was not detected in any of the samples. BRSV was detected in diseased calves in two herds but not in the clinically healthy animals. Among the diseased calves in these two herds a significant increase in haptoglobin and serum amyloid A levels was observed compared to the healthy calves. The results indicate that haptoglobin might be the best choice for detecting disease under field conditions. For H. somni and M. haemolytica, a higher percentage of the samples were found positive by PCR than by cultivation, whereas the opposite result was found for P. multocida. Detection of P. multocida by PCR or cultivation was found to be significantly associated with the disease status of the calves. For H. somni a similar association with disease status was only observed for cultivation and not for PCR.
Url:
DOI: 10.1016/j.vetmic.2008.12.024
PubMed: 19186010
Affiliations:
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Le document en format XML
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Heegaard</name></author><author><name sortKey="Enemark, Jorg M D" sort="Enemark, Jorg M D" uniqKey="Enemark J" first="Jörg M D" last="Enemark">Jörg M D. Enemark</name></author></analytic><series><title level="j">Veterinary microbiology</title><idno type="ISSN">0378-1135</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute-Phase Proteins (metabolism)</term><term>Animals</term><term>Bacterial Infections (diagnosis)</term><term>Bacterial Infections (veterinary)</term><term>Bronchoalveolar Lavage Fluid (microbiology)</term><term>Cattle</term><term>Cattle Diseases (diagnosis)</term><term>Cattle Diseases (microbiology)</term><term>Cattle Diseases (virology)</term><term>Haptoglobins (metabolism)</term><term>Lung (microbiology)</term><term>Lung (virology)</term><term>Pneumonia (diagnosis)</term><term>Pneumonia (microbiology)</term><term>Pneumonia (veterinary)</term><term>Pneumonia (virology)</term><term>Polymerase Chain Reaction (veterinary)</term><term>Serum Amyloid A Protein (metabolism)</term><term>Trachea</term><term>Virus Diseases (diagnosis)</term><term>Virus Diseases (veterinary)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Haptoglobines (métabolisme)</term><term>Infections bactériennes (diagnostic)</term><term>Infections bactériennes (médecine vétérinaire)</term><term>Liquide de lavage bronchoalvéolaire (microbiologie)</term><term>Maladies des bovins (diagnostic)</term><term>Maladies des bovins (microbiologie)</term><term>Maladies des bovins (virologie)</term><term>Maladies virales (diagnostic)</term><term>Maladies virales (médecine vétérinaire)</term><term>Pneumopathie infectieuse (diagnostic)</term><term>Pneumopathie infectieuse (microbiologie)</term><term>Pneumopathie infectieuse (médecine vétérinaire)</term><term>Pneumopathie infectieuse (virologie)</term><term>Poumon (microbiologie)</term><term>Poumon (virologie)</term><term>Protéine amyloïde A sérique (métabolisme)</term><term>Protéine de la phase aigüe (métabolisme)</term><term>Réaction de polymérisation en chaîne (médecine vétérinaire)</term><term>Trachée</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acute-Phase Proteins</term><term>Haptoglobins</term><term>Serum Amyloid A Protein</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bacterial Infections</term><term>Cattle Diseases</term><term>Pneumonia</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections bactériennes</term><term>Maladies des bovins</term><term>Maladies virales</term><term>Pneumopathie infectieuse</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Liquide de lavage bronchoalvéolaire</term><term>Maladies des bovins</term><term>Pneumopathie infectieuse</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bronchoalveolar Lavage Fluid</term><term>Cattle Diseases</term><term>Lung</term><term>Pneumonia</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections bactériennes</term><term>Maladies virales</term><term>Pneumopathie infectieuse</term><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Haptoglobines</term><term>Protéine amyloïde A sérique</term><term>Protéine de la phase aigüe</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Bacterial Infections</term><term>Pneumonia</term><term>Polymerase Chain Reaction</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des bovins</term><term>Pneumopathie infectieuse</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cattle Diseases</term><term>Lung</term><term>Pneumonia</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Trachea</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Trachée</term></keywords><keywords scheme="mix" xml:lang="it"><term>Medicine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during September and November 2002. The 90 samples were cultivated and investigated by PCR tests targeting the species Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, Mycoplasma dispar, and Mycoplasma bovirhinis. A PCR test amplifying the lktC-artJ intergenic region was evaluated and shown to be specific for the two species M. haemolytica and Mannheimia glucosida. All 90 aspirations were also analyzed for bovine respiratory syncytial virus (BRSV), parainfluenza-3 virus, and bovine corona virus by antigen ELISA. Surprisingly, 63% of the apparently healthy calves harbored potentially pathogenic bacteria in the lower respiratory tract, 60% of these samples contained either pure cultures or many pathogenic bacteria in mixed culture. Among diseased calves, all samples showed growth of pathogenic bacteria in the lower respiratory tract. All of these were classified as pure culture or many pathogenic bacteria in mixed culture. A higher percentage of the samples were positive for all bacterial species in the group of diseased animals compared to the clinically healthy animals, however this difference was only significant for M. dispar and M. bovirhinis. M. bovis was not detected in any of the samples. BRSV was detected in diseased calves in two herds but not in the clinically healthy animals. Among the diseased calves in these two herds a significant increase in haptoglobin and serum amyloid A levels was observed compared to the healthy calves. The results indicate that haptoglobin might be the best choice for detecting disease under field conditions. For H. somni and M. haemolytica, a higher percentage of the samples were found positive by PCR than by cultivation, whereas the opposite result was found for P. multocida. Detection of P. multocida by PCR or cultivation was found to be significantly associated with the disease status of the calves. For H. somni a similar association with disease status was only observed for cultivation and not for PCR.</div></front></TEI><affiliations><list><country><li>Danemark</li></country></list><tree><noCountry><name sortKey="Enemark, Jorg M D" sort="Enemark, Jorg M D" uniqKey="Enemark J" first="Jörg M D" last="Enemark">Jörg M D. Enemark</name><name sortKey="Heegaard, Peter M H" sort="Heegaard, Peter M H" uniqKey="Heegaard P" first="Peter M H" last="Heegaard">Peter M H. Heegaard</name><name sortKey="Kokotovic, Branko" sort="Kokotovic, Branko" uniqKey="Kokotovic B" first="Branko" last="Kokotovic">Branko Kokotovic</name><name sortKey="Larsen, Jesper" sort="Larsen, Jesper" uniqKey="Larsen J" first="Jesper" last="Larsen">Jesper Larsen</name><name sortKey="Larsen, Lars Erik" sort="Larsen, Lars Erik" uniqKey="Larsen L" first="Lars Erik" last="Larsen">Lars Erik Larsen</name><name sortKey="Thomsen, John" sort="Thomsen, John" uniqKey="Thomsen J" first="John" last="Thomsen">John Thomsen</name></noCountry><country name="Danemark"><noRegion><name sortKey="Angen, Oystein" sort="Angen, Oystein" uniqKey="Angen O" first="Oystein" last="Angen">Oystein Angen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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